TYPES OF PCR

REVERSE TRANSCRIPTION PCR

  • It is employed for amplification of RNA molecules.
  • RT-PCR is widely used in expression profiling to determine the expression of a gene or to identify the sequence of RNA transcript.


QUANTITATIVE REAL-TIME PCR


It used to amplify and also for quantification and detection of DNA sample:

  1. Real time PCR using DNA dyes.
  2. Flourescent report probe method

Detection and quantification of flourescent reporter the signal of which increases in direct proportion to the amount of PCR product in a reaction. Does not measure the amount of end product but it production in real time.


 

APPLICATION



  1. Forensic DNA analysis
  2. Paternity testing and determining family relationship
  3. Human remains identification
  4. Diagnostic tests
  5. DNA cloning
  6. Human genetic testing
  7. Amplification of rare DNA
  8. Engineered microbe for production of protein or enzyme to speed up the industrial processes.